Dual Photoredox Catalysis Strategy for Enhanced Photopolymerization-Based Colorimetric Biodetection.
ACS Appl Mater Interfaces
; 13(48): 57962-57970, 2021 Dec 08.
Article
em En
| MEDLINE
| ID: mdl-34797978
Catalytic redox reactions have been employed to enhance colorimetric biodetection signals in point-of-care diagnostic tests, while their time-sensitive visual readouts may increase the risk of false results. To address this issue, we developed a dual photocatalyst signal amplification strategy that can be controlled by a fixed light dose, achieving time-independent colorimetric biodetection in paper-based tests. In this method, target-associated methylene blue (MB+) photocatalytically amplifies the concentration of eosin Y by oxidizing deactivated eosin Y (EYH3-) under red light, followed by photopolymerization with eosin Y autocatalysis under green light to generate visible hydrogels. Using the insights from mechanistic studies on MB+-sensitized photo-oxidation of EYH3-, we improved the photocatalytic efficiency of MB+ by suppressing its degradation. Lastly, we characterized 100- to 500-fold enhancement in sensitivity obtained from MB+-specific eosin Y amplification, highlighting the advantages of using dual photocatalyst signal amplification.
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Base de dados:
MEDLINE
Assunto principal:
Colorimetria
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Amarelo de Eosina-(YS)
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Materiais Biomiméticos
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Azul de Metileno
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article