The potential for isotope dilution-LC-MS/MS to improve laboratory measurement of C-peptide: Reasons and critical determinants.
J Mass Spectrom Adv Clin Lab
; 21: 1-9, 2021 Aug.
Article
em En
| MEDLINE
| ID: mdl-34820671
ABSTRACT
Human C-peptide is secreted in equimolar amounts with insulin by pancreatic beta-cells. Measurement of C-peptide plays an important role in the diagnosis and treatment of diabetes where it is used to evaluate the function of islet cells. However, C-peptide measurement results across different laboratories vary considerably and there is an urgent need to improve comparability between laboratories. As it is sensitive and specific, isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) has made a major contribution and will continue to play a significant role in the standardization of C-peptide measurement. Here, we reviewed the application of ID-LC-MS/MS in C-peptide measurement by discussing the biochemical properties of C-peptide, common sample preparation procedures, and the sensitivity problems often encountered with ID-LC-MS/MS C-peptide measurement. Collectively, these factors are crucial for the development of ID-LC-MS/MS methods for C-peptide measurement. We also discussed the advantages, disadvantages, and progress of implementing ID-LC-MS/MS as a routine measurement tool for C-peptide in clinical laboratories. Finally, we summarized the existing reference system and the status of C-peptide measurement in clinical laboratories to convey the necessity of improving the comparability of C-peptide measurement in clinical laboratories using ID-LC-MS/MS.
%CV, coefficient of variation expressed as a percent; C-peptide; EAQ, external quality assessment program; ID-LC-MS/MS, isotope dilution-liquid chromatography-tandem mass spectrometry; Isotope dilution liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS); JCTLM, the Joint Committee for Traceability in Laboratory Medicine; PI, The isoelectric point; Sample preparation; Standardization
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MEDLINE
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En
Ano de publicação:
2021
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Article