[Effect of swimming training on the expression of PKC δ/p66Shc protein in mouse myocardium].
Zhongguo Ying Yong Sheng Li Xue Za Zhi
; 37(6): 688-693, 2021 Nov.
Article
em Zh
| MEDLINE
| ID: mdl-34821107
ABSTRACT
Objective:
To investigate the effects of different intensity of swimming training on p66Shc protein in mouse myocardium.Methods:
Fifty Kunming mice were randomly divided into control group (Group C), weight-bearing swimming group (Group E), weight-bearing swimming + drug group (Group ER), non weight-bearing swimming group (Group P), non weight-bearing swimming + drug group (Group PR), with 10 mice in each group. Group C did not exercise. Groups E, ER, P, and PR received swimming training for 4 weeks. Groups E and ER performed weight-bearing swimming with a 3% body weight, and Group P and Group PR were swimming without weight-bearing, 60 min/d, 6 times/w. Mice in ER and PR groups were injected intraperitoneally with Rottlerin (0.3 mg/kg), a PKCδ inhibitor, before the last two exercises. Groups C, E, and P were injected with the same dose of normal saline. Samples were collected after training finished for 24 hours. The protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 were detected by Western blot; PKCδ and P66Shc were detected by immunoprecipitation; malondialdehyde (MDA), reactive oxygen species (ROS) and superoxide dismutase (SOD) in myocardium and serum were analyzed by biochemistry.Results:
Compared with Group C, the protein expressions of PKCδ, P-PKCδ, P66Shc, P-P66shc and NOX2 in Group E were increased significantly (Pï¼ 0.01), the serum and myocardial MDA levels, myocardial ROS were increased significantly (Pï¼0.05 or Pï¼0.01), and the myocardial SOD activity was decreased (Pï¼0.01), the PKCδ, P-PKCδ, P-P66shc and NOX2 in Group P were increased significantly (Pï¼0.05 or Pï¼0.01), and the myocardial SOD activity was enhanced (Pï¼0.05). Compared with Group E, the protein expressionS of PKCδ (Pï¼0.01), P-PKCδ (Pï¼0.01), P66Shc (Pï¼0.05), P-P66shc (Pï¼0.01), NOX2 (Pï¼0.05) in Group ER was decreased significantly, the protein expression of P66Shc in Group P was decreased significantly (Pï¼0.05), the myocardial MDA (Pï¼0.01) and ROS (Pï¼0.05) were decreased, and the activity of SOD was enhanced (Pï¼0.01). Compared with Group P, the protein expressions of PKCδ, P-PKCδ and P-P66shc in Group PR were decreased significantly (Pï¼0.01), while the expression of NOX2 was increased (Pï¼0.05).Conclusion:
Both swimming training of two intensities promoted the increase of PKCδ protein and its phosphorylation in mouse cardiomyocytes. High-intensity swimming training could significantly enhance the expression and phosphorylation level of p66Shc protein, resulting in the production of ROS and the decrease of antioxidant enzyme activity. Low-intensity swimming training enhanced the phosphorylation of p66Shc, but did not promote its protein expression, resulting in the enhancement of myocardial antioxidant capacity and exercise adaptation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Condicionamento Físico Animal
/
Proteína Quinase C-delta
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Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src
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Miocárdio
Idioma:
Zh
Ano de publicação:
2021
Tipo de documento:
Article