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Siderophore-mediated zinc acquisition enhances enterobacterial colonization of the inflamed gut.
Behnsen, Judith; Zhi, Hui; Aron, Allegra T; Subramanian, Vivekanandan; Santus, William; Lee, Michael H; Gerner, Romana R; Petras, Daniel; Liu, Janet Z; Green, Keith D; Price, Sarah L; Camacho, Jose; Hillman, Hannah; Tjokrosurjo, Joshua; Montaldo, Nicola P; Hoover, Evelyn M; Treacy-Abarca, Sean; Gilston, Benjamin A; Skaar, Eric P; Chazin, Walter J; Garneau-Tsodikova, Sylvie; Lawrenz, Matthew B; Perry, Robert D; Nuccio, Sean-Paul; Dorrestein, Pieter C; Raffatellu, Manuela.
Afiliação
  • Behnsen J; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Zhi H; Department of Microbiology & Immunology, University of Illinois Chicago, Chicago, IL, USA.
  • Aron AT; Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA.
  • Subramanian V; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.
  • Santus W; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, CA, 92093, USA.
  • Lee MH; University of Kentucky PharmNMR Center, College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0596, USA.
  • Gerner RR; Department of Microbiology & Immunology, University of Illinois Chicago, Chicago, IL, USA.
  • Petras D; Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA.
  • Liu JZ; Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA.
  • Green KD; Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, CA, USA.
  • Price SL; Collaborative Mass Spectrometry Innovation Center, University of California, San Diego, La Jolla, CA, 92093, USA.
  • Camacho J; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Hillman H; Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0596, USA.
  • Tjokrosurjo J; Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
  • Montaldo NP; Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA.
  • Hoover EM; Division of Host-Microbe Systems & Therapeutics, Department of Pediatrics, University of California San Diego, La Jolla, CA, 92093, USA.
  • Treacy-Abarca S; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Gilston BA; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Skaar EP; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Chazin WJ; Department of Microbiology & Molecular Genetics, University of California Irvine, Irvine, CA, USA.
  • Garneau-Tsodikova S; Department of Biochemistry and Chemistry, and Center for Structural Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Lawrenz MB; Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Perry RD; Department of Biochemistry and Chemistry, and Center for Structural Biology, Vanderbilt University Medical Center, Nashville, TN, USA.
  • Nuccio SP; Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, 40536-0596, USA.
  • Dorrestein PC; Center for Predictive Medicine for Biodefense and Emerging Infectious Diseases, Department of Microbiology and Immunology, University of Louisville School of Medicine, Louisville, KY, 40202, USA.
  • Raffatellu M; Department of Microbiology and Immunology, University of Kentucky, Lexington, KY, 40536, USA.
Nat Commun ; 12(1): 7016, 2021 12 01.
Article em En | MEDLINE | ID: mdl-34853318
ABSTRACT
Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or "Nissle") exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin's affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Zinco / Sideróforos / Enterobacteriaceae Idioma: En Ano de publicação: 2021 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Zinco / Sideróforos / Enterobacteriaceae Idioma: En Ano de publicação: 2021 Tipo de documento: Article