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Combined effect of cell geometry and polarity domains determines the orientation of unequal division.
Godard, Benoit G; Dumollard, Remi; Heisenberg, Carl-Philipp; McDougall, Alex.
Afiliação
  • Godard BG; Laboratoire de Biologie du Développement de Villefranche-sur-mer, Institut de la Mer de Villefranche-sur-mer, Sorbonne Université, CNRS, Villefranche sur Mer, France.
  • Dumollard R; Institute of Science and Technology Austria, Klosterneuburg, Austria.
  • Heisenberg CP; Laboratoire de Biologie du Développement de Villefranche-sur-mer, Institut de la Mer de Villefranche-sur-mer, Sorbonne Université, CNRS, Villefranche sur Mer, France.
  • McDougall A; Institute of Science and Technology Austria, Klosterneuburg, Austria.
Elife ; 102021 12 10.
Article em En | MEDLINE | ID: mdl-34889186
ABSTRACT
Cell division orientation is thought to result from a competition between cell geometry and polarity domains controlling the position of the mitotic spindle during mitosis. Depending on the level of cell shape anisotropy or the strength of the polarity domain, one dominates the other and determines the orientation of the spindle. Whether and how such competition is also at work to determine unequal cell division (UCD), producing daughter cells of different size, remains unclear. Here, we show that cell geometry and polarity domains cooperate, rather than compete, in positioning the cleavage plane during UCDs in early ascidian embryos. We found that the UCDs and their orientation at the ascidian third cleavage rely on the spindle tilting in an anisotropic cell shape, and cortical polarity domains exerting different effects on spindle astral microtubules. By systematically varying mitotic cell shape, we could modulate the effect of attractive and repulsive polarity domains and consequently generate predicted daughter cell size asymmetries and position. We therefore propose that the spindle position during UCD is set by the combined activities of cell geometry and polarity domains, where cell geometry modulates the effect of cortical polarity domain(s).
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Urocordados / Divisão Celular / Polaridade Celular / Forma Celular / Desenvolvimento Embrionário / Embrião não Mamífero Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Urocordados / Divisão Celular / Polaridade Celular / Forma Celular / Desenvolvimento Embrionário / Embrião não Mamífero Idioma: En Ano de publicação: 2021 Tipo de documento: Article