HUMANIZED MODEL OF ISOLATED SUSPENSION CULTIVATION OF HEMATOPOIETIC PROGENITOR CELLS FOR THE INVESTIGATION OF IONIZING RADIATION INFLUENCE IN VIVO. / GUMANIZOVANA MODEL' KUL'TYVUVANNIa IZOL'OVANOÏ SUSPENZIÏ GEMOPOETYChNYKh KLITYN/POPEREDNYTs' DLIa VYVChENNIa VPLYVU IONIZUIuChOÏ RADIATsIÏ IN VIVO.

ABSTRACT

OBJECTIVE: development of the humanized system for cells cultivation outside the human organism (human-mouse)and investigation of the influence of ionizing radiation in increasing doses on the colony-forming ability ofhematopoietic progenitor cells. MATERIALS AND METHODS: Bone marrow samples of individuals without blood system diseases were cultivated in geldiffusion chambers with semi-solid agar in the abdominal cavity of CBA mice exposed to ionizing radiation action.Cell aggregates, which were obtained in the culture of diffusion chambers in vivo, were counted and colony-formingefficiency of bone marrow cells was determined. RESULTS: We revealed the stimulation of colony forming under the action of ionizing radiation in increasing doseson the animals-recipients of the chambers, which indirectly indicates the synthesis of colony-stimulating factor inthe mice organism and its permeation into the diffusion chambers with human bone marrow cells. The effect of cyto-statics action on the mice organism was investigated, which in experimentally selected dose cause stimulation ofcolony forming in cell cultures, both 24 hours and 2 hours after administration. CONCLUSIONS: The ability of hematopoietic progenitor cells of bone marrow to form colonies and clusters was eval-uated during the cultivation in semi-solid agar in gel diffusion chambers in vivo, as well as the association with thenumber of explanted cells in the appropriate range was established, which indicates the clonal nature of cell aggre-gates growth in culture. It was shown that the treatment of animals the day prior to experiment with administra-tion of cytostatics is comparable to the action of ionizing radiation and can be used to study hematopoiesis in«human-mouse¼ system.