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Detection of Antibody Responses Against SARS-CoV-2 in Plasma and Saliva From Vaccinated and Infected Individuals.
Klingler, Jéromine; Lambert, Gregory S; Itri, Vincenza; Liu, Sean; Bandres, Juan C; Enyindah-Asonye, Gospel; Liu, Xiaomei; Simon, Viviana; Gleason, Charles R; Kleiner, Giulio; Chiu, Hsin-Ping; Hung, Chuan-Tien; Kowdle, Shreyas; Amanat, Fatima; Lee, Benhur; Zolla-Pazner, Susan; Upadhyay, Chitra; Hioe, Catarina E.
Afiliação
  • Klingler J; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Lambert GS; James J. Peters VA Medical Center, Bronx, NY, United States.
  • Itri V; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Liu S; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Bandres JC; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Enyindah-Asonye G; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Liu X; Infectious Diseases Section, James J. Peters VA Medical Center, Bronx, NY, United States.
  • Simon V; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Gleason CR; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Kleiner G; James J. Peters VA Medical Center, Bronx, NY, United States.
  • Chiu HP; Division of Infectious Diseases, Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Hung CT; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Kowdle S; Department of Pathology, Molecular and Cell Based Medicine Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Amanat F; Global Health and Emerging Pathogen Institute, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Lee B; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Zolla-Pazner S; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Upadhyay C; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
  • Hioe CE; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States.
Front Immunol ; 12: 759688, 2021.
Article em En | MEDLINE | ID: mdl-34987505
Antibodies (Abs) are essential for the host immune response against SARS-CoV-2, and all the vaccines developed so far have been designed to induce Abs targeting the SARS-CoV-2 spike. Many studies have examined Ab responses in the blood from vaccinated and infected individuals. However, since SARS-CoV-2 is a respiratory virus, it is also critical to understand the mucosal Ab responses at the sites of initial virus exposure. Here, we examined plasma versus saliva Ab responses in vaccinated and convalescent patients. Although saliva levels were significantly lower, a strong correlation was observed between plasma and saliva total Ig levels against all SARS-CoV-2 antigens tested. Virus-specific IgG1 responses predominated in both saliva and plasma, while a lower prevalence of IgM and IgA1 Abs was observed in saliva. Antiviral activities of plasma Abs were also studied. Neutralization titers against the initial WA1 (D614G), B.1.1.7 (alpha) and B.1.617.2 (delta) strains were similar but lower against the B.1.351 (beta) strain. Spike-specific antibody-dependent cellular phagocytosis (ADCP) activities were also detected and the levels correlated with spike-binding Ig titers. Interestingly, while neutralization and ADCP potencies of vaccinated and convalescent groups were comparable, enhanced complement deposition to spike-specific Abs was noted in vaccinated versus convalescent groups and corresponded with higher levels of IgG1 plus IgG3 among the vaccinated individuals. Altogether, this study demonstrates the detection of Ab responses after vaccination or infection in plasma and saliva that correlate significantly, although Ig isotypic differences were noted. The induced plasma Abs displayed Fab-mediated and Fc-dependent functions with comparable neutralization and ADCP potencies, but a greater capacity to activate complement was elicited upon vaccination.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saliva / Anticorpos Neutralizantes / Glicoproteína da Espícula de Coronavírus / Vacinas contra COVID-19 / SARS-CoV-2 / COVID-19 / Anticorpos Antivirais Idioma: En Ano de publicação: 2021 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saliva / Anticorpos Neutralizantes / Glicoproteína da Espícula de Coronavírus / Vacinas contra COVID-19 / SARS-CoV-2 / COVID-19 / Anticorpos Antivirais Idioma: En Ano de publicação: 2021 Tipo de documento: Article