Molecular cloning and expression analysis of mytilin-like antimicrobial peptides from Asian green mussel Perna viridis.
Fish Shellfish Immunol
; 121: 239-244, 2022 Feb.
Article
em En
| MEDLINE
| ID: mdl-34990807
ABSTRACT
Mytilin is one of the most important CS-αß peptides involved in innate immune response in Mytilidae. In this study, we successfully identified four mytilin-like antimicrobial peptides (pernalins) from Asian green mussel Perna viridis by aligning the P. viridis transcriptome with 186 mytilins and myticins related sequences collected from the transcriptome data of six Mytilus species. Analysis on gene structure showed that pernalin genes had high conservation with mytilin B of Mediterranean mussel Mytilus galloprovincialis. Interestingly, all pernalin genes have a similar tissue expression feature, evidenced by the highest transcription level observed in the hemocytes and followed by the mantle. The lowest transcription level was observed in the foot and gills. qRT-PCR analysis showed that all pernalin genes were significantly down-regulated at each time points from 3 h to 48 h after Vibrio parahaemolyticus infection, suggesting their timely immune responses after bacterial infection.
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Base de dados:
MEDLINE
Assunto principal:
Mytilus
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Perna (Organismo)
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Peptídeos Antimicrobianos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article