Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence.
Methods Mol Biol
; 2435: 107-127, 2022.
Article
em En
| MEDLINE
| ID: mdl-34993942
ABSTRACT
We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies.
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Base de dados:
MEDLINE
Assunto principal:
Microambiente Tumoral
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article