Your browser doesn't support javascript.
loading
RNA sequencing of chronic GVHD skin lesions defines shared and unique inflammatory pathways characterizing lichen planus and morphea.
Zouali, Habib; Lemasson, Juliette; Calugareanu, Andreea; Battail, Christophe; Michonneau, David; le Buanec, Hélène; Grolleau, Chloé; Cassius, Charles; Robin, Marie; Merandet, Marine; Dobos, Gabor; Mahevas, Thibault; Rybojad, Michel; de Masson, Adèle; Amode, Reyhan; Boland, Anne; Michel, Laurence; Sicre de Fontbrune, Flore; Peffault de Latour, Régis; Bruneval, Patrick; Ait-Oufella, Hafid; Battistella, Maxime; Jachiet, Marie; Bagot, Martine; Deleuze, Jean-François; Socié, Gérard; Bouaziz, Jean-David.
Afiliação
  • Zouali H; Fondation Jean Dausset-Centre d'Etude du Polymorphisme Humain (CEPH), Paris, France.
  • Lemasson J; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Calugareanu A; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Battail C; Université de Paris, Paris, France.
  • Michonneau D; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • le Buanec H; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Grolleau C; University Grenoble Alpes, Interdisciplinary Research Institute of Grenoble (IRIG), Laboratoire Biologie et Biotechnologies pour la Santé, UMR 1292 INSERM, Grenoble, France.
  • Cassius C; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Robin M; Centre National de Recherche en Génomique Humaine (CNRGH), Institut François Jacob, CEA, Université Paris Saclay, Evry, France.
  • Merandet M; Haematology Transplantation, Hôpital Saint-Louis, AP-HP, Paris, France.
  • Dobos G; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Mahevas T; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Rybojad M; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • de Masson A; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Amode R; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Boland A; Université de Paris, Paris, France.
  • Michel L; Haematology Transplantation, Hôpital Saint-Louis, AP-HP, Paris, France.
  • Sicre de Fontbrune F; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Peffault de Latour R; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Bruneval P; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Ait-Oufella H; Université de Paris, Paris, France.
  • Battistella M; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Jachiet M; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Bagot M; Department of Dermatology, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.
  • Deleuze JF; Université de Paris, Paris, France.
  • Socié G; INSERM U976, Hôpital Saint-Louis, Paris, France.
  • Bouaziz JD; Centre National de Recherche en Génomique Humaine (CNRGH), Institut François Jacob, CEA, Université Paris Saclay, Evry, France.
Blood Adv ; 6(9): 2805-2811, 2022 05 10.
Article em En | MEDLINE | ID: mdl-35008096
Cutaneous involvement of chronic graft-versus-host disease (cGVHD) has a wide range of manifestations including a lichenoid form with a currently assumed mixed Th1/Th17 signature and a sclerotic form with Th1 signature. Despite substantial heterogeneity of innate and adaptive immune cells recruited to the skin and of the different clinical manifestations, treatment depends mainly on the severity of the skin involvement and relies on systemic, high-dose glucocorticoids alone or in combination with a calcineurin inhibitor. We performed the first study using RNA sequencing to profile and compare the transcriptome of lichen planus cGVHD (n = 8), morphea cGVHD (n = 5), and healthy controls (n = 6). Our findings revealed shared and unique inflammatory pathways to each cGVHD subtype that are both pathogenic and targetable. In particular, the deregulation of IFN signaling pathway was strongly associated with cutaneous cGVHD, whereas the triggering receptor expressed on myeloid cells 1 pathway was found to be specific of lichen planus and likely contributes to its pathogenesis. The results were confirmed at a protein level by performing immunohistochemistry staining and at a transcriptomic level using real-time quantitative polymerase chain reaction.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Esclerodermia Localizada / Doença Enxerto-Hospedeiro / Líquen Plano Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Esclerodermia Localizada / Doença Enxerto-Hospedeiro / Líquen Plano Idioma: En Ano de publicação: 2022 Tipo de documento: Article