Identification of traditional Japanese Kampo medicines and crude drugs that upregulate brainderived neurotrophic factor in human peripheral cells.
Acta Neurobiol Exp (Wars)
; 81(4): 393-404, 2021.
Article
em En
| MEDLINE
| ID: mdl-35014988
ABSTRACT
The neurotrophic hypothesis of depression, which suggests that decreased hippocampal brainderived neurotrophic factor (BDNF) levels cause depression, has become increasingly popular. BDNF, a member of the neurotrophin family, promotes neuronal differentiation and survival. BDNF is synthesized in various peripheral tissues, as well as in the brain. Considering that peripheral BDNF can be transported into the brain across the bloodbrain barrier, substances with the ability to upregulate BDNF activity in peripheral tissues may be useful in the management of depression. Previously, we demonstrated that the human kidney adenocarcinoma cell line ACHN produces BDNF; hence, this cell line was employed for screening upregulators of peripheral BDNF. Here, we aimed to identify Kampo (traditional Japanese) medicines and their crude drug components that upregulate BDNF levels using ACHN cells. Chotosan, Hochuekkito, Kososan, and Ninjinyoeito, Kampo medicines used in treating psychiatric disorders, increased BDNF levels in the culture media of ACHN cells. Furthermore, Chinpi (Citrus unshiu peel), a crude drug contained in these four Kampo medicines, as well as Onji (Polygala tenuifolia root), and Saiko (Bupleurum falcatum root) elevated BDNF levels in ACHN cells. Chinpi, showing strong BDNF elevating effect, increased BDNF mRNA expression. Inhibitors of protein kinase B, mitogenactivated protein kinase kinase, and cAMPdependent protein kinase, involved in the transcription of BDNF, attenuated Chinpiinduced BDNF elevation. Our results suggest that Chinpi and Kampo medicines containing Chinpi can promote the production of BDNF in peripheral tissues, potentially alleviating depression symptoms.
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Base de dados:
MEDLINE
Assunto principal:
Fator Neurotrófico Derivado do Encéfalo
/
Medicina Kampo
Idioma:
En
Ano de publicação:
2021
Tipo de documento:
Article