Oil-Free Acoustofluidic Droplet Generation for Multicellular Tumor Spheroid Culture.
ACS Appl Bio Mater
; 2(9): 4097-4105, 2019 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-35021343
ABSTRACT
We present an easy-to-assemble microfluidic system for synthesizing cell-loaded dextran/alginate (DEX/ALG) hydrogel spheres using an aqueous two-phase system (ATPS) for templated fabrication of multicellular tumor spheroids (MTSs). An audio speaker driven by an amplified output of a waveform generator or smartphone provides acoustic modulation to drive the breakup of an ATPS into MTS template droplets within microcapillary fluidic devices. We apply extensions of Plateau-Rayleigh theory to help define the flow and frequency parameter space necessary for acoustofluidic ATPS droplet formation in these devices. This method provides a simple droplet microfluidic approach using off-the-shelf acoustic components for quickly initiating MTSs and subsequent 3D cell culture.
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MEDLINE
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En
Ano de publicação:
2019
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Article