Cryosectioning.

Ross, Mark A; Kohut, Lauryn; Loughran, Patricia A

Cryosectioning.

Ross, Mark A; Kohut, Lauryn; Loughran, Patricia A.

Afiliação

Ross MA; University of Pittsburgh, Center for Biologic Imaging, Pittsburgh, Pennsylvania.
Kohut L; University of Pittsburgh, Department of Surgery, Pittsburgh, Pennsylvania.
Loughran PA; University of Pittsburgh, Center for Biologic Imaging, Pittsburgh, Pennsylvania.

Curr Protoc ; 2(1): e342, 2022 Jan.

Article em En | MEDLINE | ID: mdl-35038380

ABSTRACT

ABSTRACT

The protocols presented here describe steps for cryosectioning tissue samples to be used in light microscopy methodologies including histochemistry, enzyme immunohistochemistry, and immunofluorescence. © 2022 Wiley Periodicals LLC. Basic Protocol 1 Cryosectioning.

Assuntos

Crioultramicrotomia; Imunofluorescência; Imuno-Histoquímica; Fixação de Tecidos

Palavras-chave

cryopreservation; cryosectioning and immunofluorescence; tissue perfusion-fixation

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Crioultramicrotomia Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Crioultramicrotomia Idioma: En Ano de publicação: 2022 Tipo de documento: Article