Protein-Protein Communication Mediated by an Antibody-Responsive DNA Nanodevice.
Angew Chem Int Ed Engl
; 61(12): e202115680, 2022 03 14.
Article
em En
| MEDLINE
| ID: mdl-35043562
ABSTRACT
We report here the rational design and optimization of an antibody-responsive, DNA-based device that enables communication between pairs of otherwise non-interacting proteins. The device is designed to recognize and bind a specific antibody and, in response, undergo a conformational change that leads to the release of a DNA strand, termed the "translator," that regulates the activity of a downstream target protein. As proof of principle, we demonstrate antibody-induced control of the proteins thrombin and Taq DNA polymerase. The resulting strategy is versatile and, in principle, can be easily adapted to control protein-protein communication in artificial regulatory networks.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Trombina
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article