Silencing of Nrf genes in the human placenta as measured by SDS-PAGE and Western Blotting techniques.
Placenta
; 118: 70-74, 2022 02.
Article
em En
| MEDLINE
| ID: mdl-35045361
ABSTRACT
Nuclear factor erythroid 2-related factor-2 (Nrf2), and the less well characterised proteins Nrf1 and Nrf3, are member of the cap 'n' collar family of transcription factors. Nrf proteins regulate the expression of endogenous antioxidant enzymes and have recently become the targets for various therapeutic treatments. Recently, Nrf proteins have been of particular interest as a target in placental-derived oxidative stress induced pregnancy disorders. Here, we report the presence of Nrf1, Nrf2 and Nrf3 proteins in both human primary trophoblast and human trophoblast choriocarcinoma cell line (BeWo). We also detail the steps taken to successfully silence all Nrf proteins in both human primary trophoblast cells and BeWo via detection of mRNA and protein using quantitative PCR, and SDS-PAGE and Western Blotting respectively.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Trofoblastos
/
Interferência de RNA
/
Fatores de Transcrição de Zíper de Leucina Básica
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article