Knockout of ABC Transporter ABCG4 Gene Confers Resistance to Cry1 Proteins in Ostrinia furnacalis.
Toxins (Basel)
; 14(1)2022 01 12.
Article
em En
| MEDLINE
| ID: mdl-35051029
ABSTRACT
Ostrinia furnacalis is an important borer on maize. Long-term and large-scale planting of transgenic corn has led O. furnacalis evolving resistance and reducing the control effect. Recently, high levels of resistance to Bt Cry1 toxins have been reported to be genetically linked to the mutation or down-regulation of ABC transporter subfamily G gene ABCG4 in O. furnacalis. In order to further determine the relationship between ABCG4 gene and the resistance to Cry1 toxins in O. furnacalis, the novel CRISPR/Cas9 genome engineering system was utilized to successfully construct ABCG4-KO knockout homozygous strain. Bioassay results indicated that an ABCG4-KO strain had a higher resistance to Cry1 proteins compared with a susceptible strain (ACB-BtS). The result indicates that the ABCG4 gene may act as a receptor of the Bt Cry1 toxin in O. furnacalis. Furthermore, the development time was significantly changed in the early stage ABCG4-KO larvae, and the population parameters were also significantly changed. In summary, our CRISPR/Cas9-mediated genome editing study presents evidence that ABCG4 gene is a functional receptor for Bt Cry1 toxins, laying the foundation for further clarification of the Bt resistance mechanism.
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Base de dados:
MEDLINE
Assunto principal:
Resistência a Inseticidas
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Proteínas de Insetos
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Endotoxinas
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Subfamília G de Transportadores de Cassetes de Ligação de ATP
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Toxinas de Bacillus thuringiensis
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Proteínas Hemolisinas
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Mariposas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article