Bleaching-Resistant Super-Resolution Fluorescence Microscopy.
Adv Sci (Weinh)
; 9(9): e2101817, 2022 03.
Article
em En
| MEDLINE
| ID: mdl-35088584
ABSTRACT
Photobleaching is the permanent loss of fluorescence after extended exposure to light and is a major limiting factor in super-resolution microscopy (SRM) that restricts spatiotemporal resolution and observation time. Strategies for preventing or overcoming photobleaching in SRM are reviewed developing new probes and chemical environments. Photostabilization strategies are introduced first, which are borrowed from conventional fluorescence microscopy, that are employed in SRM. SRM-specific strategies are then highlighted that exploit the on-off transitions of fluorescence, which is the key mechanism for achieving super-resolution, which are becoming new routes to address photobleaching in SRM. Off states can serve as a shelter from excitation by light or an exit to release a damaged probe and replace it with a fresh one. Such efforts in overcoming the photobleaching limits are anticipated to enhance resolution to molecular scales and to extend the observation time to physiological lifespans.
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Base de dados:
MEDLINE
Assunto principal:
Ácido Hipocloroso
/
Corantes Fluorescentes
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article