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WDFY1, a WD40 repeat protein, is not essential for spermatogenesis and male fertility in mice.
Lv, Chunyu; Xiong, Mengneng; Guo, Shuangshuang; Gui, Yiqian; Liu, Xiaohua; Wang, Xiaoli; Wu, Yanqing; Feng, Shenglei; Zhang, Jin; Zhang, Yan; Liu, Yu; Qin, Weibing; Yuan, Shuiqiao.
Afiliação
  • Lv C; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical
  • Xiong M; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Guo S; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Gui Y; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Liu X; NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital), Guangzhou, 510600, China.
  • Wang X; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Wu Y; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Feng S; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Zhang J; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Zhang Y; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
  • Liu Y; State Key Laboratory of Virology, Frontier Science Center for Immunology and Metabolism, College of Life Sciences, Wuhan University, Wuhan, 430072, China.
  • Qin W; NHC Key Laboratory of Male Reproduction and Genetics, Guangdong Provincial Reproductive Science Institute (Guangdong Provincial Fertility Hospital), Guangzhou, 510600, China. Electronic address: guardqin@163.com.
  • Yuan S; Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, Guangdong, 518057, China. Electronic address: shuiqiaoyuan@hust.edu.cn.
Biochem Biophys Res Commun ; 596: 71-75, 2022 03 12.
Article em En | MEDLINE | ID: mdl-35121371
ABSTRACT
The mouse WD repeat and FYVE domain containing 1 (Wdfy1) gene is located in chromosome 1qC4 and spans over 73.7 kilobases. It encodes a protein of 410-amino acid protein that shares 97.8% amino acid sequence identity with the human WDFY1 protein. However, the expression pattern of WDFY1 in reproductive organs and its function in male fertility remain unknown. In this study, we generated transgenic mice expressing FLAG-Wdfy1-mCherry cDNA driven by the Wdfy1 promoter to clarify the expression of WDFY1. The results showed that WDFY1 is highly expressed in mouse testes and located in the cytoplasm of late pachytene spermatocytes to elongated spermatids. Interestingly, the global Wdfy1 knockout (KO) male mice displayed normal growth, development, and fertility. Further histological analysis of Wdfy1 knockout mouse testes revealed that all spermatogenic cells are present in Wdfy1 KO seminiferous tubules. Together, our data demonstrate that WDFY1 is dispensable for mouse spermatogenesis and male fertility.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatogênese / Testículo / Regulação da Expressão Gênica / Proteínas Adaptadoras de Transdução de Sinal / Fertilidade Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Espermatogênese / Testículo / Regulação da Expressão Gênica / Proteínas Adaptadoras de Transdução de Sinal / Fertilidade Idioma: En Ano de publicação: 2022 Tipo de documento: Article