Proteomic analysis revealed the biofilm-degradation abilities of the bacteriophage UPMK_1 and UPMK_2 against Methicillin-resistant Staphylococcus aureus.
Biotechnol Lett
; 44(3): 513-522, 2022 Mar.
Article
em En
| MEDLINE
| ID: mdl-35122191
ABSTRACT
OBJECTIVE:
The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography.METHODS:
The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed.RESULTS:
UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host.CONCLUSION:
Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Bacteriófagos
/
Staphylococcus aureus Resistente à Meticilina
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article