Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept.
Sci Rep
; 12(1): 2356, 2022 02 18.
Article
em En
| MEDLINE
| ID: mdl-35181702
ABSTRACT
Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Saliva
/
Programas de Rastreamento
/
Espectroscopia de Luz Próxima ao Infravermelho
/
Exsudatos e Transudatos
/
SARS-CoV-2
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article