Optical nanotopography of fluorescent surfaces by axial position modulation.
Opt Express
; 30(4): 6425-6439, 2022 Feb 14.
Article
em En
| MEDLINE
| ID: mdl-35209581
ABSTRACT
We present an optical method that combines confocal microscopy with position modulation to perform axial tracking and topographic imaging of fluorescent surfaces. Using a remote focusing system, the confocal observation volume is oscillated in the axial direction. The resulting modulation of the detected signal is used as a feedback to precisely control the distance to an object of interest. The accuracy of this method is theoretically analyzed and the axial-locking accuracy is experimentally evaluated. Topographic imaging is demonstrated on fluorescently coated beads and fixed cells. This microscope allows for nanometric topography or tracking of dynamic fluorescent surfaces.
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MEDLINE
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En
Ano de publicação:
2022
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Article