Physically interacting beta-delta pairs in the regenerating pancreas revealed by single-cell sequencing.
Mol Metab
; 60: 101467, 2022 06.
Article
em En
| MEDLINE
| ID: mdl-35240340
ABSTRACT
OBJECTIVES:
Until recently, communication between neighboring cells in islets of Langerhans was overlooked by genomic technologies, which require rigorous tissue dissociation into single cells.METHODS:
We utilize sorting of physically interacting cells (PICs) with single-cell RNA-sequencing to systematically map cellular interactions in the endocrine pancreas after pancreatectomy.RESULTS:
The pancreas cellular landscape features pancreatectomy associated heterogeneity of beta-cells, including an interaction-specific program between paired beta and delta-cells.CONCLUSIONS:
Our analysis suggests that the particular cluster of beta-cells that pairs with delta-cells benefits from stress protection, implying that the interaction between beta- and delta-cells might safeguard against pancreatectomy associated challenges. The work encourages testing the potential relevance of physically-interacting beta-delta-cells also in diabetes mellitus.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ilhotas Pancreáticas
/
Células Secretoras de Insulina
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article