High-resolution imaging of the mouse-hair-cell hair bundle by scanning electron microscopy.
STAR Protoc
; 3(1): 101213, 2022 03 18.
Article
em En
| MEDLINE
| ID: mdl-35257116
ABSTRACT
Scanning electron microscopy (SEM) allows cell surface imaging at a sub-nanometric resolution. However, the sample requires a specific preparation to sustain the high vacuum of the SEM and be electrically conductive. The sample preparation consists of dissection, fixation, dehydration, metal coating, and tissue mounting. Here we provide a comprehensive protocol to perform SEM on the mouse's inner ear, and image the hair bundles at high resolution. Hair bundles are the force-sensitive organelles located at the apical surface of hair cells. For complete details on the use and execution of this protocol, please refer to Trouillet et al. (2021).
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Células Ciliadas Auditivas
/
Orelha Interna
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article