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Structural and Biochemical Basis for Development of Diketo Acid Inhibitors Targeting the Cap-Snatching Endonuclease of the Ebinur Lake Virus (Order: Bunyavirales).
Kuang, Wenhua; Zhang, Huanyu; Cai, Yan; Zhang, Guilin; Deng, Fei; Li, Hailong; Zhou, Yiwu; Wang, Manli; Gong, Peng; Guo, Yu; Hu, Zhihong.
Afiliação
  • Kuang W; Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
  • Zhang H; State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences, Wuhan, Hubei, China.
  • Cai Y; State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences, Wuhan, Hubei, China.
  • Zhang G; State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, China.
  • Deng F; Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, China.
  • Li H; Center for Disease Control and Prevention of Xinjiang Military Command, Urumqi, Xinjiang, China.
  • Zhou Y; State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences, Wuhan, Hubei, China.
  • Wang M; Center for Disease Control and Prevention of Xinjiang Military Command, Urumqi, Xinjiang, China.
  • Gong P; Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
  • Guo Y; State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences, Wuhan, Hubei, China.
  • Hu Z; Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, Hubei, China.
J Virol ; 96(7): e0217321, 2022 04 13.
Article em En | MEDLINE | ID: mdl-35266805
The Bunyavirales contain many important human pathogens that lack an antiviral therapy. The cap-snatching endonuclease (EN) of segmented negative-strand RNA viruses is an attractive target for broad-spectrum antivirals due to its essential role in initiating viral transcription. L-742,001, a previously reported diketo acid inhibitor against influenza virus EN, demonstrated potent EN inhibition and antiviral activity on various bunyaviruses. However, the precise inhibitory mechanism of the compound is still poorly understood. We recently characterized a highly active EN from Ebinur Lake virus (EBIV), a newly identified member of the Orthobunyavirus genus, and obtained its high-resolution structures, paving the way for structure-guided inhibitor development. Here, nine L-742,001 derivatives were designed and synthesized de novo, and their structure-activity relationship with EBIV EN was studied. In vitro biochemical data showed that the compounds inhibited the EBIV EN activity with different levels and could be divided into three categories. Five representative compounds were selected for further cell-based antiviral assay, and the results largely agreed with those of the EN assays. Furthermore, the precise binding modes of L-742,001 and its derivatives in EN were revealed by determining the high-resolution crystal structures of EN-inhibitor complexes, which suggested that the p-chlorobenzene is essential for the inhibitory activity and the flexible phenyl has the greatest exploration potential. This study provides an important basis for the structure-based design and optimization of inhibitors targeting EN of segmented negative-strand RNA viruses. IMPORTANCE The Bunyavirales contain many important human pathogens such as Crimean-Congo hemorrhagic fever virus and Lassa virus that pose serious threats to public health; however, currently there are no specific antiviral drugs against these viruses. The diketo acid inhibitor L-742,001 is a potential drug as it inactivates the cap-snatching endonuclease (EN) encoded by bunyaviruses. Here, we designed and synthesized nine L-742,001 derivatives and assessed the structure-activity relationship using EN of the newly identified Ebinur Lake virus (EBIV) as a research model. Our results revealed that the p-chlorobenzene of this broad-spectrum EN inhibitor is crucial for the inhibitory activity and the flexible phenyl "arm" has the best potential for further optimization. As cap-snatching ENs are present not only in bunyaviruses but also in influenza viruses, our data provide important guidelines for the development of novel and more potent diketo acid-based antiviral drugs against those viruses.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antivirais / Proteínas Virais / Bunyaviridae / Endonucleases Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Antivirais / Proteínas Virais / Bunyaviridae / Endonucleases Idioma: En Ano de publicação: 2022 Tipo de documento: Article