Medium-chain fatty acids production from carbohydrates-rich wastewater through two-stage yeast biofilm processes without external electron donor addition: Biofilm development and pH impact.

The production of medium-chain fatty acids (MCFAs) is considered promising for carbon resource recovery from waste streams. However, a large quantity of external electron donors are often required, causing great cost and environmental impact. Therefore, in this study, a two-stage technology was developed to produce MCFAs from carbohydrate-rich wastewater without external electron donor addition, with the biofilm development and pH impact being explored. Stage I aimed at converting organics into ethanol and a yeast biofilm reactor is innovatively applied. The results showed that the yeast biofilm could quickly form on carriers with steady-state thickness reaching 50-200 µm. However, the attachment of yeast biofilm was weak at the initial stage so that the violent turbulence should be avoided during operation. The polyurethane foam was the most suitable for yeast biofilm development among the tested carriers, as evidenced by the highest ethanol production, accounting for 74.2% of soluble organics. The Nakaseomyces was the main fungal genus in the steady-state biofilm, while lactic acid bacteria were also developed, resulting in lactate and acetate production. In Stage II, the yeast biofilm reactor effluent was applied for MCFA production at different pH (5-8). However, the MCFA production selectivity was significantly affected by pH, with 65.2% at pH of 5 but decreasing substantially to 3.0% at pH of 8. Both the microbial and electron transfer efficiency analysis suggested that mildly acidic pH can promote the electron transfer from ethanol toward the chain elongation process instead of its excessive oxidation. Thus, if conditions of online extraction or microbial tolerance permit, a lower pH should be recommended for Stage II in the developed technology as well as other ethanol-based MCFA production process. This is a conceptual study that eliminated external electron donor addition in MCFAs production and provide a sustainable and reliable way in carbon resources recovery.