Intratesticular xenografting of Klinefelter pre-pubertal testis tissue as potential model to study testicular fibrosis.
Reprod Biomed Online
; 44(5): 896-906, 2022 05.
Article
em En
| MEDLINE
| ID: mdl-35282995
ABSTRACT
RESEARCH QUESTION Is intratesticular xenotransplantation a potential ex-vivo model for studying testicular fibrosis related to Klinefelter syndrome? STUDY DESIGN:
First, a feasibility study of an ex-vivo model to study testicular fibrosis in patients with Klinefelter syndrome was performed. Testis tissue from boys with pre-pubertal Klinefelter syndrome (nâ¯=â¯3) and controls (nâ¯=â¯2) (<18 years) was grafted to the mouse testis (nâ¯=â¯12) and recovered after 2, 4, 6 and 8 weeks. Part two of this study consisted of a validation of this model, evaluating the effects of the mast cell blocker ketotifen on the histology of the grafts of Klinefelter syndrome (nâ¯=â¯5) and controls (nâ¯=â¯3), transplanted to mice (nâ¯=â¯10), after 4 weeks of ketotifen or saline treatment. Immunohistochemistry determined the histology of the grafts and the presence of mast cells and spermatogonia.RESULTS:
The feasibility study showed that 4 weeks after transplantation, all Klinefelter syndrome grafts could be recovered. Later, degeneration was observed. Most recovered grafts showed an intact histology, with 67 ± 12% intact tubules for the Klinefelter syndrome grafts and 65 ± 15% of intact tubules for the control grafts. In the few remaining Klinefelter syndrome grafts, treatment with ketotifen improved testicular histology compared with non-treated grafts. Graft survival was patient dependent. No germ cell loss was observed after transplantation.CONCLUSION:
Xenografting could become a model for the longitudinal study of the fibrotic process related to Klinefelter syndrome; however, the current model has a limited survival period and patient-specific differences in histology.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Síndrome de Klinefelter
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article