Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.
Cell
; 185(7): 1208-1222.e21, 2022 03 31.
Article
em En
| MEDLINE
| ID: mdl-35305314
ABSTRACT
The tumor microenvironment hosts antibody-secreting cells (ASCs) associated with a favorable prognosis in several types of cancer. Patient-derived antibodies have diagnostic and therapeutic potential; yet, it remains unclear how antibodies gain autoreactivity and target tumors. Here, we found that somatic hypermutations (SHMs) promote antibody antitumor reactivity against surface autoantigens in high-grade serous ovarian carcinoma (HGSOC). Patient-derived tumor cells were frequently coated with IgGs. Intratumoral ASCs in HGSOC were both mutated and clonally expanded and produced tumor-reactive antibodies that targeted MMP14, which is abundantly expressed on the tumor cell surface. The reversion of monoclonal antibodies to their germline configuration revealed two types of classes one dependent on SHMs for tumor binding and a second with germline-encoded autoreactivity. Thus, tumor-reactive autoantibodies are either naturally occurring or evolve through an antigen-driven selection process. These findings highlight the origin and potential applicability of autoantibodies directed at surface antigens for tumor targeting in cancer patients.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ovarianas
/
Anticorpos Antineoplásicos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article