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Clr-f expression regulates kidney immune and metabolic homeostasis.
Zein, Haggag S; Abou-Samra, Elias; Scur, Michal; Gutsol, Alex; Hall, Clayton W; Dasgupta, Bishal; Gharibeh, Lara; Abujamel, Turki; Medina-Luna, Daniel; Gamage, Gayani S; Pelino, Tessa J; Nemer, Mona; Rahim, Mir Munir A; Steinle, Alexander; Parsons, Brendon D; Makrigiannis, Andrew P.
Afiliação
  • Zein HS; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Abou-Samra E; King Abdullah International Medical Research Centre, Immunology Research Program, Riyadh, Saudi Arabia.
  • Scur M; Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON, Canada.
  • Gutsol A; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Hall CW; Division of Nephrology, Department of Medicine, Kidney Research Centre, Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.
  • Dasgupta B; Department of Pathology and Molecular Medicine, Division of Medical Microbiology, McMaster University, Hamilton, ON, Canada.
  • Gharibeh L; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Abujamel T; Molecular Genetics and Cardiac Regeneration Laboratory, Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON, Canada.
  • Medina-Luna D; Division of Cardiac Surgery, University of Ottawa Heart Institute, Ottawa, ON, Canada.
  • Gamage GS; Vaccines and Immunotherapy Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Pelino TJ; Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • Nemer M; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Rahim MMA; Beatrice Hunter Cancer Research Institute, Halifax, NS, Canada.
  • Steinle A; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Parsons BD; Department of Microbiology and Immunology, Dalhousie University, Halifax, NS, Canada.
  • Makrigiannis AP; Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, ON, Canada.
Sci Rep ; 12(1): 4834, 2022 03 22.
Article em En | MEDLINE | ID: mdl-35318366
ABSTRACT
The C-type lectin-related protein, Clr-f, encoded by Clec2h in the mouse NK gene complex (NKC), is a member of a family of immune regulatory lectins that guide immune responses at distinct tissues of the body. Clr-f is highly expressed in the kidney; however, its activity in this organ is unknown. To assess the requirement for Clr-f in kidney health and function, we generated a Clr-f-deficient mouse (Clr-f-/-) by targeted deletions in the Clec2h gene. Mice lacking Clr-f exhibited glomerular and tubular lesions, immunoglobulin and C3 complement protein renal deposits, and significant abdominal and ectopic lipid accumulation. Whole kidney transcriptional profile analysis of Clr-f-/- mice at 7, 13, and 24 weeks of age revealed a dynamic dysregulation in lipid metabolic processes, stress responses, and inflammatory mediators. Examination of the immune contribution to the pathologies of Clr-f-/- mouse kidneys identified elevated IL-12 and IFNγ in cells of the tubulointerstitium, and an infiltrating population of neutrophils and T and B lymphocytes. The presence of these insults in a Rag1-/-Clr-f-/- background reveals that Clr-f-/- mice are susceptible to a T and B lymphocyte-independent renal pathogenesis. Our data reveal a role for Clr-f in the maintenance of kidney immune and metabolic homeostasis.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Matadoras Naturais / Lectinas Tipo C Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células Matadoras Naturais / Lectinas Tipo C Idioma: En Ano de publicação: 2022 Tipo de documento: Article