3-Azi-1-methoxybutyl D-maltooligosaccharides specifically bind to the maltose/maltooligosaccharide-binding protein of Escherichia coli and can be used as photoaffinity labels.
Eur J Biochem
; 160(1): 83-91, 1986 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-3533533
ABSTRACT
Maltooligosaccharides with two to six (alpha 1-4)-linked glucose residues, carrying at their reducing end a 3-azi-1-methoxybutyl group in either alpha or in beta glycosidic linkage, were synthesized. These maltooligosaccharide analogues inhibit maltose uptake via the maltose-binding-protein-dependent transport system in Escherichia coli. The concentration of half-maximal inhibition of maltose transport, at 15 nM concentration, decreases with increasing chain length of the analogue, levelling off at 40 microM after a chain length of four glucose residues in the alpha series and at 350 microM after a chain length of three glucose residues in the beta series. The inhibition of maltose transport occurs at the level of the periplasmic maltose-binding protein. 3-Azi-1-methoxybutyl alpha-D-[3H]maltotrioside was bound by the maltose-binding protein with a Kd of 0.18 mM. Irradiation at 350 nm of purified maltose-binding protein in the presence of 4 microM of this substrate labeled the protein covalently; labeling was prevented by 1 mM maltose. Using a crude preparation of periplasmic proteins two proteins were labeled, the maltose-binding protein and alpha-amylase. Thus, 3-azi-1-methoxybutyl alpha-D-maltooligosaccharides are potent photoaffinity labels for proteins with maltooligosaccharides-binding sites.
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Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Azidas
/
Proteínas de Bactérias
/
Proteínas de Transporte de Monossacarídeos
/
Marcadores de Afinidade
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Proteínas de Transporte
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Transportadores de Cassetes de Ligação de ATP
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Proteínas de Escherichia coli
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Proteínas Periplásmicas de Ligação
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Escherichia coli
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article