A novel system for the generation of baculoviruses mutant for an essential gene.

Su, Wujie; Qu, Jianwei; Ren, Yuying; Wang, Wenbing; Li, Fanchi; Li, Bing

Su, Wujie; Qu, Jianwei; Ren, Yuying; Wang, Wenbing; Li, Fanchi; Li, Bing.

Afiliação

Su W; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China.
Qu J; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China.
Ren Y; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China.
Wang W; Department of Preventive Medicine and Public Health Laboratory Science, School of Medicine, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu, China.
Li F; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China.
Li B; School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, 215123, Jiangsu, People's Republic of China. lib@suda.edu.cn.

Mol Biol Rep ; 49(7): 6443-6452, 2022 Jul.

Article em En | MEDLINE | ID: mdl-35430685

Assuntos

Bombyx; Nucleopoliedrovírus; Animais; Baculoviridae/genética; Bombyx/genética; Escherichia coli/genética; Genes Essenciais/genética; Vetores Genéticos; Nucleopoliedrovírus/genética

Palavras-chave

Bm92; BmNPV bac-to-bac expression system; Gene knockout; Purification; Transfer vector construction; orf92

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bombyx / Nucleopoliedrovírus Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Bombyx / Nucleopoliedrovírus Idioma: En Ano de publicação: 2022 Tipo de documento: Article