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Fluconazole-resistant Candida parapsilosis clonally related genotypes: first report proving the presence of endemic isolates harbouring the Y132F ERG11 gene substitution in Spain.
Alcoceba, Eva; Gómez, Ana; Lara-Esbrí, Paula; Oliver, Antonio; Beltrán, Adrián Ferre; Ayestarán, Ignacio; Muñoz, Patricia; Escribano, Pilar; Guinea, Jesús.
Afiliação
  • Alcoceba E; Clinical Microbiology Department, Hospital Universitari Son Espases, Mallorca, Spain.
  • Gómez A; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Lara-Esbrí P; Clinical Microbiology Department, Hospital Universitari Son Espases, Mallorca, Spain.
  • Oliver A; Clinical Microbiology Department, Hospital Universitari Son Espases, Mallorca, Spain; CIBER de Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain.
  • Beltrán AF; Infectious Diseases Unit, Hospital Universitari Son Espases, Mallorca, Spain.
  • Ayestarán I; Intensive Care Unit, Hospital Universitari Son Espases, Mallorca, Spain.
  • Muñoz P; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain; Medicine Department, Faculty of Medicine, Unive
  • Escribano P; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Guinea J; Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain; CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain. Electronic address: jguineaortega@yahoo.es.
Clin Microbiol Infect ; 28(8): 1113-1119, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35439634
OBJECTIVES: We report here for the first time the presence of Candida parapsilosis isolates harbouring the Y132F ERG11 gene substitution in patients admitted to a Spanish hospital. METHODS: We studied the available (n = 104) C parapsilosis isolates from patients admitted to the Son Espases reference hospital in the Balearic Islands from 1 April 2019 to 30 November 2020. Isolates were sourced from 70 patients: catheter (n = 41), blood cultures (n = 37), lower respiratory tract (n = 15), intra-abdominal (n = 8), and other samples (n = 3). Isolates were genotyped and tested for antifungal susceptibilities to amphotericin B, triazoles, anidulafungin, and micafungin using EUCAST E.Def 7.3.2. The ERG11 gene was sequenced in fluconazole-resistant isolates. RESULTS: Among the 104 isolates, fluconazole and voriconazole resistance was found in 87 (84%) and 30 (29%) isolates, respectively; all isolates were fully susceptible to echinocandins and amphotericin B. All fluconazole-resistant isolates harboured the Y132F substitution in the ERG11 gene and were grouped into 11 clonally related genotypes. A genotype accounted for 70% (61/87) of fluconazole-resistant isolates. Genotypes involving the fluconazole-resistant isolates were different from those found in the remaining fluconazole-susceptible genotypes. Fifty-six patients harboured fluconazole-resistant genotypes, and 35 of the 56 had candidaemia (48%), abdominal candidiasis (17%), or other forms of candidiasis (35%). Only 20% of the study patients infected by fluconazole-resistant genotypes had a history of azole use. DISCUSSION: Fluconazole resistance in C parapsilosis isolates from patients admitted to this reference hospital is not attributable to prior azole use, but rather to the presence of a group of fluconazole-resistant C parapsilosis genotypes that have become endemic.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Candidíase / Fluconazol Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Candidíase / Fluconazol Idioma: En Ano de publicação: 2022 Tipo de documento: Article