Hydrogel assisted synthesis of gold nanoparticles with enhanced microbicidal and in vivo wound healing potential.
Sci Rep
; 12(1): 6575, 2022 04 21.
Article
em En
| MEDLINE
| ID: mdl-35449438
ABSTRACT
The present study reports a hydrogel-based sunlight-assisted synthesis of gold nanoparticles (Au NPs) with enhanced antimicrobial and wound healing potential. The hydrogel extracted from the seeds of Cydonia oblonga was used as a reducing and capping agent to synthesize Au NPs for the first time. The as-synthesized Au NPs were characterized for an average size, shape, surface functionalization, antimicrobial, and wound healing capabilities. The cubic and rectangular-shaped Au NPs with an average edge length of 74 ± 4.57 nm depicted a characteristic surface plasmon resonance band at 560 nm. The hydrogel-based Au NPs inhibited the growth of microorganisms in zones with 12 mm diameter. In-vitro experiments showed that a minimum inhibitory concentration of Au NPs (16 µg/mL) was sufficient to mimic the 95% growth of pathogenic microorganisms in 24 h. In vivo treatment of wounds with Au NPs in murine models revealed a 99% wound closure within 5 days. Quantitative PCR analysis performed to decipher the role of Au NPs in enhanced wound healing showed an increase in the expression levels of NANOG and CD-34 proteins.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Nanopartículas Metálicas
/
Anti-Infecciosos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article