Fluorescence-Activated Cell Sorting and Quantitative Real-Time PCR to Reveal VEGF-Expressing Macrophage Populations in the Zebrafish Larvae.

Herman, Andrew; Greenhough, Alexander; Gurevich, David B

Herman, Andrew; Greenhough, Alexander; Gurevich, David B.

Afiliação

Herman A; Faculty of Life Sciences, School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.
Greenhough A; Centre for Research in Biosciences, Faculty of Health and Applied Sciences, University of the West of England, Bristol, UK.
Gurevich DB; Department of Biology and Biochemistry, Centre for Therapeutic Innovation, Faculty of Science, University of Bath, Bath, UK. dbg29@bath.ac.uk.

Methods Mol Biol ; 2475: 325-337, 2022.

Article em En | MEDLINE | ID: mdl-35451769

Assuntos

Fator A de Crescimento do Endotélio Vascular; Peixe-Zebra; Animais; Células Endoteliais; Citometria de Fluxo/métodos; Larva/genética; Macrófagos; Reação em Cadeia da Polimerase em Tempo Real; Fator A de Crescimento do Endotélio Vascular/genética; Peixe-Zebra/genética

Palavras-chave

FACS; Immune cells; Macrophages; Quantitative PCR; VEGF; Wounding; Zebrafish

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peixe-Zebra / Fator A de Crescimento do Endotélio Vascular Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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