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Dark-Field Microscopic Study of Cellular Uptake of Carbon Nanodots: Nuclear Penetrability.
Zhang, Wendi; Ji, Zuowei; Zeng, Zheng; Jayapalan, Anitha; Bagra, Bhawna; Sheardy, Alex; He, Peng; LaJeunesse, Dennis R; Wei, Jianjun.
Afiliação
  • Zhang W; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Ji Z; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Zeng Z; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Jayapalan A; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Bagra B; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Sheardy A; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • He P; Department of Chemistry, North Carolina Agricultural and Technical State University, Greensboro, NC 27411, USA.
  • LaJeunesse DR; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
  • Wei J; Department of Nanoscience, Joint School of Nanoscience and Nanoengineering, University of North Carolina at Greensboro, Greensboro, NC 27401, USA.
Molecules ; 27(8)2022 Apr 09.
Article em En | MEDLINE | ID: mdl-35458634
Carbon nanodots are fascinating candidates for the field of biomedicine, in applications such as bioimaging and drug delivery. However, the nuclear penetrability and process are rarely studied and lack understanding, which limits their applications for drug carriers, single-molecule detection and live cell imaging. In this study, we attempt to examine the uptake of CNDs in cells with a focus on the potential nuclear penetrability using enhanced dark-field microscopy (EDFM) associated with hyperspectral imaging (HSI) to quantitatively determine the light scattering signals of CNDs in the cells. The effects of both CND incubation time and concentration are investigated, and plausible nuclear penetration involving the nuclear pore complex (NPC) is discussed. The experimental results and an analytical model demonstrate that the CNDs' uptake proceeds by a concentration-dependent three-stage behavior and saturates at a CND incubation concentration larger than 750 µg/mL, with a half-saturated concentration of 479 µg/mL. These findings would potentially help the development of CNDs' utilization in drug carriers, live cell imaging and other biomedical applications.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carbono / Microscopia Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Carbono / Microscopia Idioma: En Ano de publicação: 2022 Tipo de documento: Article