Detection of muscle stem cell-derived myonuclei in murine overloaded muscles.
STAR Protoc
; 3(2): 101307, 2022 06 17.
Article
em En
| MEDLINE
| ID: mdl-35463471
ABSTRACT
Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU+ myonuclei and image acquisition for quantitative analyses. For complete details on the use and execution of this protocol, please refer to Kaneshige et al. (2022).
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Base de dados:
MEDLINE
Assunto principal:
Células Satélites de Músculo Esquelético
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article