Real-Time Imaging of Single Î³TuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy.

Consolati, Tanja; Henkin, Gil; Roostalu, Johanna; Surrey, Thomas

Consolati, Tanja; Henkin, Gil; Roostalu, Johanna; Surrey, Thomas.

Afiliação

Consolati T; The Francis Crick Institute, London, UK.
Henkin G; Centre for Genomic Regulation (CRG), Barcelona Institute of Science and Technology (BIST), Barcelona, Spain.
Roostalu J; Pharmaceutical and Regulatory Services GmbH (PRSG), Graz, Austria.
Surrey T; The Francis Crick Institute, London, UK.

Methods Mol Biol ; 2430: 315-336, 2022.

Article em En | MEDLINE | ID: mdl-35476342

Assuntos

Centrossomo; Proteínas Associadas aos Microtúbulos; Microscopia; Proteínas Associadas aos Microtúbulos/química; Centro Organizador dos Microtúbulos; Microtúbulos/química

Palavras-chave

Functionalized glass surface; Gammatubulin ring complex; In vitro assay; Microtubule dynamics; Microtubule nucleation; Protein immobilization; Single molecule imaging; TIRF microscopy

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Centrossomo / Proteínas Associadas aos Microtúbulos Idioma: En Ano de publicação: 2022 Tipo de documento: Article

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