Generation of Thermally Stable Affinity Pairs for Sensitive, Specific Immunoassays.
Methods Mol Biol
; 2491: 417-469, 2022.
Article
em En
| MEDLINE
| ID: mdl-35482202
ABSTRACT
Many point-of-care diagnostic tests rely on a pair of monoclonal antibodies that bind to two distinct epitopes of a molecule of interest. This protocol describes the identification and generation of such affinity pairs based on an easily produced small protein scaffold rcSso7d which can substitute monoclonal antibodies. These strong binding variants are identified from a large yeast display library. The approach described can be significantly faster than antibody generation and epitope binning, yielding affinity pairs synthesized in common bacterial protein synthesis strains, enabling the rapid generation of novel diagnostic tools.
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Base de dados:
MEDLINE
Assunto principal:
Anticorpos Monoclonais
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article