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Co-Electrospun Silk Fibroin and Gelatin Methacryloyl Sheet Seeded with Mesenchymal Stem Cells for Tendon Regeneration.
Xue, Yumeng; Kim, Han-Jun; Lee, Junmin; Liu, Yaowen; Hoffman, Tyler; Chen, Yi; Zhou, Xingwu; Sun, Wujin; Zhang, Shiming; Cho, Hyun-Jong; Lee, JiYong; Kang, Heemin; Ryu, WonHyoung; Lee, Chang-Moon; Ahadian, Samad; Dokmeci, Mehmet R; Lei, Bo; Lee, KangJu; Khademhosseini, Ali.
Afiliação
  • Xue Y; State Key Laboratory of Solidification Processing, Center for Nano Energy Materials, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, 710072, China.
  • Kim HJ; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Lee J; Frontier Institute of Science and Technology, Xi'an Jiaotong University, Xi'an, 710054, China.
  • Liu Y; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Hoffman T; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA.
  • Chen Y; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Zhou X; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA.
  • Sun W; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Zhang S; College of Food Science, Sichuan Agricultural University, Yaan, 625014, China.
  • Cho HJ; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Lee J; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Kang H; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Ryu W; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Lee CM; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA.
  • Ahadian S; Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT), University of California, Los Angeles, CA, 90095, USA.
  • Dokmeci MR; Terasaki Institute for Biomedical Innovation, Los Angeles, CA, 90064, USA.
  • Lei B; College of Pharmacy, Kangwon National University, Chuncheon, 24341, Republic of Korea.
  • Lee K; Department of Mechanical Engineering, Yonsei University, Seoul, 03722, Republic of Korea.
  • Khademhosseini A; Department of Materials Science and Engineering, Korea University, Seoul, 02841, Republic of Korea.
Small ; 18(21): e2107714, 2022 05.
Article em En | MEDLINE | ID: mdl-35487761
ABSTRACT
Silk fibroin (SF) is a promising biomaterial for tendon repair, but its relatively rigid mechanical properties and low cell affinity have limited its application in regenerative medicine. Meanwhile, gelatin-based polymers have advantages in cell attachment and tissue remodeling but have insufficient mechanical strength to regenerate tough tissue such as tendons. Taking these aspects into account, in this study, gelatin methacryloyl (GelMA) is combined with SF to create a mechanically strong and bioactive nanofibrous scaffold (SG). The mechanical properties of SG nanofibers can be flexibly modulated by varying the ratio of SF and GelMA. Compared to SF nanofibers, mesenchymal stem cells (MSCs) seeded on SG fibers with optimal composition (SG7) exhibit enhanced growth, proliferation, vascular endothelial growth factor production, and tenogenic gene expression behavior. Conditioned media from MSCs cultured on SG7 scaffolds can greatly promote the migration and proliferation of tenocytes. Histological analysis and tenogenesis-related immunofluorescence staining indicate SG7 scaffolds demonstrate enhanced in vivo tendon tissue regeneration compared to other groups. Therefore, rational combinations of SF and GelMA hybrid nanofibers may help to improve therapeutic outcomes and address the challenges of tissue-engineered scaffolds for tendon regeneration.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanofibras / Células-Tronco Mesenquimais / Fibroínas Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Nanofibras / Células-Tronco Mesenquimais / Fibroínas Idioma: En Ano de publicação: 2022 Tipo de documento: Article