Chemical modification of tryptophanase from E. coli with polyethylene glycol to reduce its immunoreactivity towards anti-tryptophanase antibodies.
Enzyme
; 36(4): 261-5, 1986.
Article
em En
| MEDLINE
| ID: mdl-3552656
ABSTRACT
Escherichia coli tryptophanase was modified with 2,4-bis(O-methoxypolyethylene glycol)-6-chloro-s-triazine (activated PEG2, MW 5,000 x 2). The modified tryptophanase, in which approximately 43% of the total 120 amino groups and 38% of the total 16 sulfhydryl groups in the molecule were coupled, completely lost the immunoreactivity towards anti-tryptophanase serum from rabbit. Approximately 10% of the enzymic activity was retained. The modified enzyme showed the same physicochemical properties as the native enzyme Km value for L-tryptophan (0.3 mmol/l), optimum pH (8.0) and optimum temperature (50 degrees C). The modified enzyme was more resistant than the native counterpart against proteolytic digestion with trypsin.
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Base de dados:
MEDLINE
Assunto principal:
Polietilenoglicóis
/
Triptofanase
/
Escherichia coli
/
Soros Imunes
/
Liases
Idioma:
En
Ano de publicação:
1986
Tipo de documento:
Article