A label-free RTP sensor based on aptamer/quantum dot nanocomposites for cytochrome c detection.
RSC Adv
; 9(55): 31953-31959, 2019 Oct 07.
Article
em En
| MEDLINE
| ID: mdl-35530815
ABSTRACT
Given the outstanding room-temperature phosphorescence (RTP) of Mn-ZnS quantum dots (QDs) and the specific recognition performance of the aptamer, we built phosphorescent composites from aptamers conjugated with polyethyleneimine quantum dots (PEI-QDs) and applied them to cytochrome c (Cyt c) detection. Specifically, QDs/CBA composites were generated from the electrostatic interaction between the positively-charged PEI-QDs and the negatively-charged Cyt c binding aptamer (CBA). With the presence of Cyt c, the Cyt c can specifically bind with the QDs/CBA composites, and quench the RTP of QDs through photoinduced electron-transfer (PIET). Thereby, an optical biosensor for Cyt c detection was built, which had a detection range of 0.166-9.96 µM and a detection limit of 0.084 µM. This aptamer-mediated phosphorescent sensor with high specificity and operational simplicity can effectively avoid the interference of scattering light from complex substrates. Our findings offer a new clue for building biosensors based on QDs and aptamers.
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MEDLINE
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Ano de publicação:
2019
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Article