Spatial epitranscriptomics reveals A-to-I editome specific to cancer stem cell microniches.
Nat Commun
; 13(1): 2540, 2022 05 09.
Article
em En
| MEDLINE
| ID: mdl-35534484
ABSTRACT
Epitranscriptomic features, such as single-base RNA editing, are sources of transcript diversity in cancer, but little is understood in terms of their spatial context in the tumour microenvironment. Here, we introduce spatial-histopathological examination-linked epitranscriptomics converged to transcriptomics with sequencing (Select-seq), which isolates regions of interest from immunofluorescence-stained tissue and obtains transcriptomic and epitranscriptomic data. With Select-seq, we analyse the cancer stem cell-like microniches in relation to the tumour microenvironment of triple-negative breast cancer patients. We identify alternative splice variants, perform complementarity-determining region analysis of infiltrating T cells and B cells, and assess adenosine-to-inosine base editing in tumour tissue sections. Especially, in triple-negative breast cancer microniches, adenosine-to-inosine editome specific to different microniche groups is identified.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Adenosina Desaminase
/
Neoplasias de Mama Triplo Negativas
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article