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Generation of homozygous PRKN, PINK1 and double PINK1/PRKN knockout cell lines from healthy induced pluripotent stem cells using CRISPR/Cas9 editing.
Chen, Carol X-Q; You, Zhipeng; Abdian, Narges; Sirois, Julien; Shlaifer, Irina; Tabatabaei, Mahdieh; Boivin, Marie-Noëlle; Gaborieau, Lydiane; Karamchandani, Jason; Beitel, Lenore K; Fon, Edward A; Durcan, Thomas M.
Afiliação
  • Chen CX; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • You Z; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • Abdian N; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • Sirois J; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • Shlaifer I; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • Tabatabaei M; C-BIG Repository (C-BIG), Montreal Neurological Institute-Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
  • Boivin MN; C-BIG Repository (C-BIG), Montreal Neurological Institute-Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
  • Gaborieau L; C-BIG Repository (C-BIG), Montreal Neurological Institute-Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
  • Karamchandani J; C-BIG Repository (C-BIG), Montreal Neurological Institute-Hospital, McGill University, Montreal, QC H3A 2B4, Canada.
  • Beitel LK; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada.
  • Fon EA; McGill Parkinson Program and Neurodegenerative Diseases Group, Montreal Neurological Institute, Department of Neurology and Neurosurgery, Montreal, QC H3A 2B4, Canada.
  • Durcan TM; The Neuro's Early Drug Discovery Unit (EDDU), McGill University, 3801 University Street, Montreal, QC H3A 2B4, Canada. Electronic address: thomas.durcan@mcgill.ca.
Stem Cell Res ; 62: 102806, 2022 07.
Article em En | MEDLINE | ID: mdl-35561458
ABSTRACT
Autosomal recessive mutations in either PRKN or PINK1 are associated with early-onset Parkinson's disease. The corresponding proteins, PRKN, an E3 ubiquitin ligase, and the mitochondrial serine/threonine-protein kinase PINK1 play a role in mitochondrial quality control. Using CRISPR/CAS9 technology we generated three human iPSC lines from the well characterized AIW002-02 control line. These isogenic iPSCs contain homozygous knockouts of PRKN (PRKN-KO, CBIGi001-A-1), PINK1 (PINK1-KO, CBIGi001-A-2) or both PINK1 and PRKN (PINK1-KO/PRKN-KO, CBIGi001-A-3). The knockout lines display normal karyotypes, express pluripotency markers and upon differentiation into relevant brain cells or midbrain organoids may be valuable tools to model Parkinson's disease.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Células-Tronco Pluripotentes Induzidas Idioma: En Ano de publicação: 2022 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Doença de Parkinson / Células-Tronco Pluripotentes Induzidas Idioma: En Ano de publicação: 2022 Tipo de documento: Article