Fate mapping of neural stem cell niches reveals distinct origins of human cortical astrocytes.
Science
; 376(6600): 1441-1446, 2022 06 24.
Article
em En
| MEDLINE
| ID: mdl-35587512
ABSTRACT
Progenitors of the developing human neocortex reside in the ventricular and outer subventricular zones (VZ and OSVZ, respectively). However, whether cells derived from these niches have similar developmental fates is unknown. By performing fate mapping in primary human tissue, we demonstrate that astrocytes derived from these niches populate anatomically distinct layers. Cortical plate astrocytes emerge from VZ progenitors and proliferate locally, while putative white matter astrocytes are morphologically heterogeneous and emerge from both VZ and OSVZ progenitors. Furthermore, via single-cell sequencing of morphologically defined astrocyte subtypes using Patch-seq, we identify molecular distinctions between VZ-derived cortical plate astrocytes and OSVZ-derived white matter astrocytes that persist into adulthood. Together, our study highlights a complex role for cell lineage in the diversification of human neocortical astrocytes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Astrócitos
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Neocórtex
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Nicho de Células-Tronco
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Neurogênese
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Células-Tronco Neurais
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article