Rituximab Attenuated Lipopolysaccharide-Induced Oxidative Cytotoxicity, Apoptosis, and Inflammation in the Human Retina Cells via Modulating the TRPM2 Signaling Pathways.
Ocul Immunol Inflamm
; 30(6): 1315-1328, 2022 Aug.
Article
em En
| MEDLINE
| ID: mdl-35587813
ABSTRACT
PURPOSE:
We investigated the possible protective effects of rituximab (RTX) on LPS-induced oxidant, inflammatory, and apoptotic adverse actions via the inhibition of TRPM2 channel in the adult retinal pigment epithelial-19 (ARPE-19) cells.METHODS:
In the cultured ARPE-19 cells, we induced five main groups as control, RTX (10 µg/ml), LPS (1 µg/ml), LPS+RTX, and LPS+TRPM2 blockers (ACA or 2/APB).RESULTS:
The levels of apoptosis, cell death, mitochondrial free reactive oxygen radicals (mitROS), cytosolic ROS, lipid peroxidation, caspase -3, caspase -8, caspase -9, ADP-ribose-induced TRPM2 current density, TNF-α, IL-1ß, cytosolic free Zn2+, and Ca2+ were increased by LPS, although their levels were diminished by the treatments of RTX and TRPM2 blockers.CONCLUSIONS:
The LPS-induced mitROS, inflammatory cytokine, and apoptosis levels were modulated via TRPM2 inhibition in the human retinal epithelial cells by the RTX treatment. The RTX may be considered as a new therapeutic approach to LPS-induced human retinal epithelial cell injury.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Lipopolissacarídeos
/
Canais de Cátion TRPM
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article