Visualization of Mutant Aggregates from Clock Neurons by Agarose Gel Electrophoresis (AGERA) in Drosophila melanogaster.
Methods Mol Biol
; 2482: 373-383, 2022.
Article
em En
| MEDLINE
| ID: mdl-35610440
ABSTRACT
The clock neurons of the fruit fly Drosophila melanogaster have become a useful model for expressing misfolded protein aggregates that accumulate in several human neurodegenerative diseases. One advantage of such an approach is that the behavioral effects can be readily quantified on circadian locomotor rhythms, sleep or activity levels via automated, highly reliable and objective procedures. Therefore, a rapid assay is required to visualize whether these neurons develop aggregates. Here we describe a modified immunoblot method, agarose gel electrophoresis (AGERA) that has been optimized for resolving aggregates from fly clock neurons.
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MEDLINE
Assunto principal:
Proteínas de Drosophila
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Drosophila melanogaster
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article