Isoquercetin alleviates sleep deprivation dependent hippocampal neurons damage by suppressing NLRP3-induced pyroptosis.
Immunopharmacol Immunotoxicol
; 44(5): 766-772, 2022 Oct.
Article
em En
| MEDLINE
| ID: mdl-35620829
ABSTRACT
Purpose:
Sleep deprivation (SD) leads to memory and cognitive impairment due to damage to the hippocampus. Isoquercetin possesses neuron-protective properties. Our study aimed to investigate the effects of isoquercetin on SD-induced hippocampal neurons damage and the underlying mechanism.Materials andmethods:
Herein, the cognitive competence was evaluated by Morris water maze test after SD. The morphology of the hippocampus was observed after Nissl staining. Moreover, the level of NLRP3 was detected by Immunofluorescent staining and western blot. In vitro study, pyroptosis was tested by TUNEL assay and flow cytometry. The levels of pyroptosis-related factors were measured by western blot.Results:
The results indicated that isoquercetin improved spatial memory and prevented change of hippocampal neurons of SD mice. Moreover, SD upregulated NLRP3 level, which was downregulated by isoquercetin. Additionally, isoquercetin rescued the increase of pyroptosis and the upregulation of NLRP3, caspase-1, ASC, IL-1ß, IL-18, and GSDMD levels induced by LPS.Conclusions:
In conclusion, isoquercetin improved learning and cognitive capability of SD mice via suppressing NLRP3-induced pyroptosis of hippocampal neurons cells, suggesting that isoquercetin might be an efficacious drug for memory disorders caused by SD.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Piroptose
/
Proteína 3 que Contém Domínio de Pirina da Família NLR
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article