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Maximum depth sequencing reveals an ON/OFF replication slippage switch and apparent in vivo selection for bifidobacterial pilus expression.
Penno, Christophe; Motherway, Mary O'Connell; Fu, Yuan; Sharma, Virag; Crispie, Fiona; Cotter, Paul D; Houeix, Benoit; Joshi, Lokesh; Bottacini, Francesca; O'Dwyer, Aoife; Loughran, Gary; Atkins, John F; van Sinderen, Douwe.
Afiliação
  • Penno C; ECOBIO, Université Rennes 1, 35042, Rennes, France.
  • Motherway MO; School of Microbiology, University College Cork, Cork, T12 YT57, Ireland.
  • Fu Y; APC Microbiome Ireland, University College Cork, Cork, T12 YT57, Ireland.
  • Sharma V; IBERS, Aberystwyth University, Aberystwyth, UK.
  • Crispie F; Department of Chemical Sciences, University of Limerick, Limerick, Ireland.
  • Cotter PD; APC Microbiome Ireland, University College Cork, Cork, T12 YT57, Ireland.
  • Houeix B; Teagasc Food Research Centre, Moorepark, Fermoy, Co., Cork, P61 C996, Ireland.
  • Joshi L; APC Microbiome Ireland, University College Cork, Cork, T12 YT57, Ireland.
  • Bottacini F; Teagasc Food Research Centre, Moorepark, Fermoy, Co., Cork, P61 C996, Ireland.
  • O'Dwyer A; National University of Ireland, Galway, Ireland.
  • Loughran G; National University of Ireland, Galway, Ireland.
  • Atkins JF; Department of Biological Sciences, Munster Technological University, Cork, Ireland.
  • van Sinderen D; APC Microbiome Ireland, University College Cork, Cork, T12 YT57, Ireland.
Sci Rep ; 12(1): 9576, 2022 06 10.
Article em En | MEDLINE | ID: mdl-35688912
The human gut microbiome, of which the genus Bifidobacterium is a prevalent and abundant member, is thought to sustain and enhance human health. Several surface-exposed structures, including so-called sortase-dependent pili, represent important bifidobacterial gut colonization factors. Here we show that expression of two sortase-dependent pilus clusters of the prototype Bifidobacterium breve UCC2003 depends on replication slippage at an intragenic G-tract, equivalents of which are present in various members of the Bifidobacterium genus. The nature and extent of this slippage is modulated by the host environment. Involvement of such sortase-dependent pilus clusters in microbe-host interactions, including bacterial attachment to the gut epithelial cells, has been shown previously and is corroborated here for one case. Using a Maximum Depth Sequencing strategy aimed at excluding PCR and sequencing errors introduced by DNA polymerase reagents, specific G-tract sequences in B. breve UCC2003 reveal a range of G-tract lengths whose plasticity within the population is functionally utilized. Interestingly, replication slippage is shown to be modulated under in vivo conditions in a murine model. This in vivo modulation causes an enrichment of a G-tract length which appears to allow biosynthesis of these sortase-dependent pili. This work provides the first example of productive replication slippage influenced by in vivo conditions. It highlights the potential for microdiversity generation in "beneficial" gut commensals.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbioma Gastrointestinal / Bifidobacterium breve Idioma: En Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Microbioma Gastrointestinal / Bifidobacterium breve Idioma: En Ano de publicação: 2022 Tipo de documento: Article