Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria.
Methods Mol Biol
; 2498: 283-292, 2022.
Article
em En
| MEDLINE
| ID: mdl-35727550
ABSTRACT
The production of recombinant proteins in bacteria made possible to obtain large quantities of proteins essential for basic and applied research. Escherichia coli remains one of the organisms of choice for recombinant proteins because of its ability to grow at high density and availability of a vast catalog of cloning vectors and mutant host strains. Here, we describe the protocols for the expression of cold-adapted (hemo)globins in Escherichia coli.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Globinas
/
Escherichia coli
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article