A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX.
Methods Mol Biol
; 2497: 333-337, 2022.
Article
em En
| MEDLINE
| ID: mdl-35771455
ABSTRACT
Intracellular reactive oxygen species (ROS) act as an important signaling transductor in cells, regulating almost every aspect of cell biology. Measurements of ROS production thus, offer links between oxidative stress and cell pathophysiology. Here, we describe a simple screening assay in intact adherent cells by fluorescence microplate readers, using dihydroethidium (DHE) and MitoSOX to measure cytosolic superoxide and mitochondrial superoxide production, respectively. This assay enables a quick and reliable assessment of ROS generation in a well-controlled environment.
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Base de dados:
MEDLINE
Assunto principal:
Espécies Reativas de Oxigênio
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Superóxidos
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Estresse Oxidativo
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article