Analysis of Organization and Activity of Mitochondrial Respiratory Chain Complexes in Primary Fibroblasts Using Blue Native PAGE.
Methods Mol Biol
; 2497: 339-348, 2022.
Article
em En
| MEDLINE
| ID: mdl-35771456
ABSTRACT
Blue Native polyacrylamide gel electrophoresis (BN-PAGE) is a well-established technique for the isolation and separation of mitochondrial membrane protein complexes in a native conformation with high resolution. In combination with histochemical staining methods, BN-PAGE has been successfully used as clinical diagnostic tool for the detection of oxidative phosphorylation (OXPHOS) defects from small tissue biopsies from patients with primary mitochondrial disease. However, its application to patient-derived primary fibroblasts is difficult due to limited proliferation and high background staining. Here, we describe a rapid and convenient method to analyze the organization and activity of OXPHOS complexes from cultured skin fibroblasts.
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Base de dados:
MEDLINE
Assunto principal:
Membranas Mitocondriais
/
Fibroblastos
Idioma:
En
Ano de publicação:
2022
Tipo de documento:
Article